Reagent RVD vs Old Fashion RNA Extraction

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  • • Reagent RVD is an alternative to RNA extraction for numerous applications
    • Offers comparable RT-qPCR results to RNA extraction procedures for many applications in 5 MINUTES
    • Cost a fraction of traditional RNA extraction procedures, with fewer steps
    • Compatible with leading microbial transport medias
    • Compatible with most leading RT-qPCR kits and master mixes
    • Compatible with automated sample processing equipment
    • Reagent RVD is stored and shipped at room temperature

 

Part No. RVD001-25 (25 ml bottle)

Part No. RVD001-100 (100 ml bottle)

​USA Quality. Manufactured 100% in the USA, in a FDA registered and certified ISO13485 / ISO9001 facility

FAQ: REAGENT RVD 

  1. Is Reagent RVD compatible with automation? a. Yes. Follow the same Reagent RVD protocol with automated systems. 
  2. What’s the lowest amount of Reagent RVD and sample I can use?
    a. You can use as little as 10μL Reagent RVD + 10μL sample. However, we recommend using >10μL for most applications.
  3. What’s the optimal ratio of Reagent RVD to sample?
    a. The optimal ratio is 1:1 for coronaviruses collected in BDTM Universal Viral Transport system or Copan EswabTM from a nasopharyngeal swab. The same ratio should be used for other viral transport medias and modified (e.g., 2.5:1, 5:1, etc.) if needed.
  4. Is Reagent RVD guaranteed to work?                                                                                                               a. Yes. Reagent RVD is guaranteed to replace traditional RNA extraction when amplifying gene targets (e.g., N1, N2, etc.) from coronaviruses collected in BDTM Universal Viral Transport system or Copan EswabTM from a nasopharyngeal swab. Reagent RVD may replace RNA extraction for many other procedures. It’s the user’s responsibility to confirm the suitability of Reagent RVD for your specific application with a pilot study. The research team at Entopsis is here to help.
  5. I’ll like to use Reagent RVD in a manner that’s different than described. How should I proceed?
    a. Start by mixing Reagent RVD 1:1 with your sample; this works for most applications where the concentration of RT-qPCR inhibitors is not high. If desired results are not observed, then add more Reagent RVD relative to your sample (e.g., 2.5:1, 5:1, 10:1) and/or heat at 95°C for longer than 5 minutes (e.g., 10 minutes). The research team at Entopsis is here to help.
  6. Does Reagent RVD work with all viral transport medias?
    a. To date, Reagent RVD has been shown to work for coronaviruses collected in BDTM Universal Viral Transport system and Copan EswabTM from a nasopharyngeal swab. It’s likely Reagent RVD can replace RNA extraction when other viral transport medias are used, but please confirm the suitability of Reagent RVD for your specific application with a pilot study. The research team at Entopsis is here to help.
  7. For how long is Reagent RVD stable if properly maintained?
    a. 12 months. After that, you can confirm the functionality of Reagent RVD with a pilot study.
  8. Can Reagent RVD be used for non-viral applications?
    a. Yes, potentially. Please confirm the suitability of Reagent RVD for your specific application with a pilot study.
  9. Can Reagent RVD be used for DNA extraction?
    a. Yes, potentially. Please confirm the suitability of Reagent RVD for your specific application with a pilot study. The research team at Entopsis is here to help.
  10. Can DNase be added to Reagent RVD processed samples?
    a. Yes.
  11. Is there a benefit to heating the sample + Reagent RVD mixture at 95°C for longer than 5 minutes?
    a. There is not a significant difference between heating samples with Reagent RVD for 5 vs. 10 minutes at 95°C for coronaviruses. However, a longer heating period is often beneficial for difficult to lyse samples (e.g., toe nail samples).
  12. I cannot heat my sample + Reagent RVD to 95°C. Can I heat it at a lower temperature but for a longer period?
    a. Yes. Heating at 80~85°C for ~15 minutes is comparable to 5 minutes at 95°C.
  13. The product looks hazy, is this normal?
    a. Yes. This is normal. Be sure Reagent RVD is homogenized before use.
  14. I noticed two liquid phases with Reagent RVD, is this normal?
    a. Yes. Reagent RVD consists of 2 phases, one clear and one hazy. This is noticeable when the product is not mixed.
  15. How do I homogenize Reagent RVD before use?
    a. Simple invert the bottle a few times without creating too many bubbles. You can also pipette up / down a few times to ensure complete mixing.

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Thank you!

Carlos Nanni
National Accounts Manager
Direct - (772) 380-6700
carlos@hmepartner.com
MES, LLC

Michael Russo
Accounts Manager
Direct - (561) 972-0390
mikerusso@hmepartner.com
MES, LLC

Intended Application: (RESEARCH ONLY)

• Direct amplification of viral RNA from coronaviruses (without RNA extraction) when the nasopharyngeal or oropharyngeal sample is collected using BD™ Universal Viral Transport (UVT) System or Copan ESwab™ with Amies media within 7 days from collection.
• Reagent RVD may replace RNA extraction for many applications (e.g., life science research, veterinary pathogen research, etc.), but it must be verified by the end user.